Fig. 1
Generation of E. coli expressing OVA in different cellular compartments. a Schematic illustration of different OVA-expressing E. coli strains. For details see also Supplementary Figure 1. b WB analysis with Abs specific for OVA, AIDAc, and GroEL of indicated strain lysates. Bacterial chaperon GroEL was used as loading control and sOVA as positive control. Boxes indicate proteins of the expected size. Box with dashed line indicates a spliced protein in EC-ovaCYTO1. ECCTRL(MBP) expresses MBP without inserted OVA peptide. c WB of trichloroacetic acid concentrated culture supernatants of indicated strains with an OVA-specific Ab. d IF for OVA (red), LPS (green), and DAPI staining (blue) of indicated strains. Bar represents 5 µm
