Fig. 4
EC-ovaOM and EC-ovaSECR induce expansion of antigen-specific Tregs. a Experimental setup. 1 × 107 CFSE-labeled CD45.2 OTII cells were adoptively transferred to CD45.1 mice 1 day prior to intestinal colonization with ECCTRL or different EC-ova strains. b Representative FACS blots indicating proliferation and FoxP3 expression in OTII cells isolated from MLNs and spleens at day 20. For gating strategy see Supplementary Figure 8A. c Bar graphs represent mean ± SEM (n = 5–8 per condition) of proliferating and FoxP3-expressing OTII cells isolated from mice of indicated conditions at day 20. Statistical analysis was performed by one-way ANOVA and Dunn’s post hoc test. *p < 0.05; **p < 0.01. # represents difference compared to ECCTRL (p < 0.05) tested by Mann–Whitney U test
