Fig. 6
Quantification of OVA uptake into BMDCs. a WB for OVA and GroEL of indicated bacterial lysates and sOVA. 2 × 107 bacteria and 10 µg of sOVA corresponds to the amounts used in the experiments described in 5A that induce a similar proliferative response in OTII cells. b IF for OVA (red), LPS (green), and DAPI (blue) of BMDCs challenged with EC-ovaOM for 20 min. Bar represents 10 µm. c Quantification of bacterial uptake into BMDCs. 1.5 × 105 BMDCs grown on coverslips were challenged with 1.5 × 106 live or heat-killed EC-ovaOM and EC-ovaCYT01 for 90 min in 200 µl of medium. Bacteria were counted after IF staining for LPS (green) and DAPI (blue) (see also Supplementary Figure 9). d IF for OVA (red), LPS (green) and DAPI (blue) of HK EC-ovaOM and HK EC-ovaCYTO1. e 3 × 106 BMDCs were incubated with 3 × 109 viable EC-ovaOM or EC-ovaCYTO1, lysates derived from 1.2 × 1010 EC-ovaOM or EC-ovaCYTO1 or indicated concentrations of sOVA. After 90 min cells were washed three times and harvested. WB of cellular lysates for OVA and GroEL was performed
