Fig. 1 | Mucosal Immunology

Fig. 1

From: Pulmonary immunization with a recombinant influenza A virus vaccine induces lung-resident CD4+ memory T cells that are associated with protection against tuberculosis

Fig. 1

Pulmonary, but not systemic, immunization with PR8.p25 induces p25 CD4+ T cells in the lung. Fifty-thousand CD45.1+ P25 Tg splenic cells were adoptively transferred into naive recipient C57Bl/6 1 day prior to immunization intranasally with 20 pfu or intraperitoneally with 5 × 104 pfu of PR8.p25. IFNγ responses in the lung (a) and in the spleen (b) against influenza A virus NP and M. tuberculosis Ag85B P25 epitopes, 3 weeks after delivery of PR8.p25 intraperitoneally (black) or intranasally (gray) measured by IFNγ ELISPOT. Number of P25 CD4+ T cells in the BAL (c), lung (d), and spleen (e) determined by flow cytometry. Data presented as mean ± SEM (n = 4)

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