Fig. 3 | Mucosal Immunology

Fig. 3

From: Pulmonary immunization with a recombinant influenza A virus vaccine induces lung-resident CD4+ memory T cells that are associated with protection against tuberculosis

Fig. 3

Detection of GFP-P25 CD4+ T cell at 6 weeks after immunization with PR8.p25 using 2-photon microscopy. Fifty-thousand GFP-P25 Tg splenic cells were adoptively transferred into naive recipient C57Bl/6 mice 1 day prior to intranasal immunization with 20 pfu PR8.p25. a GFP-P25 cells (green) were detected in an ex vivo lung from mice immunized with PR8.p25 vaccine 6 weeks after immunization. b The extravascular location of the GFP-P25 cells (green) induced by immunization with PR8.p25 was confirmed after injection of WGA-A594 that allows visualization of the vasculature (red). c Detection of GFP-P25 cells (green) deeper into the tissue after clearing of the lung with RapiClear1.52. d Comparison of the frequency of GFP-P25 cells in the lung as determined by flow cytometry and by 2-P microscopy 8 weeks after immunization with PR8.p25

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