Fig. 4

P25 CD4⁺ T cells induced by PR8.p25 immunization have the transcriptional profile of tissue-resident memory cells. Fifty-thousand CD45.1⁺ P25 Tg splenic cells were adoptively transferred into naive recipient C57Bl/6 mice 1 day prior to immunization intranasally with 20 pfu PR8.p25. Lung CD69⁺ P25 CD4⁺ T cells (L-RM) and splenic CD69-CD62Llo (S-EM) were sorted from mice immunized with PR8.p25 6 weeks before (n = 12–20). P25 CD4⁺ effector cells (S-eff) were sorted from the lungs of mice 11 days after immunization with PR8.p25. Quantitative real-time PCR analysis of (a) klf2, s1pr1, foxo1, tbx21, ifng and (b) bcl-2 and bcl-xl mRNA in P25 cells sorted from the spleens and lungs of naive and PR8-p25 immunized mice. The representative figure shows the results from one of two independent experiments