Fig. 7
X31.p25-induced lung-resident CD4+ T cells confer protection against M. tuberculosis following depletion of circulating T cells. Fifty-thousand GFP-P25 Tg splenic cells were adoptively transferred into naive recipient C57Bl/6 mice 1 day prior to intranasal immunization with 5 × 104 pfu X31-p25. Naive and mice immunized with X31-p25 were treated with FTY720 and challenged with M. tuberculosis as in Fig. 5. Mycobacterial loads in the lungs of mice were determined at 17 (a) and 28 days (b) after infection with M. tuberculosis. The data are shown with symbols for individual animals and as mean ± SEM (n = 6) and are representative of results from one of two independent experiments. ****p < 0.0001,***p < 0.001, **p < 0.01, ANOVA followed by Tukey post-test correction
