Fig. 3

Increased early CCR2 ligand expression is induced in the lung following HN878 infection. a RT-PCR analysis was performed at 21 and 30 dpi to determine mRNA expression for Ccl2, Ccl7, and Ccl12 in lungs of H37Rv- or HN878-infected mice (n = 5 per group, per time point). b C10 epithelia, BMDCs, and BMDMs were cultured and infected with indicated Mtb strains at an MOI of 1 for 48 h (n = 6). Supernatants were analyzed by multiplex or ELISA assay for CCL2. c–e IKK2^fl/fl Sftpc-Cre mice and littermate controls were infected with HN878 for 14 (n = 7) and 30 (n = 5) dpi and the accumulation of c AMs, d neutrophils, and e RMs were calculated by flow cytometry. f Bacterial burden in the lung was determined by plating at 14 (n = 7 per group) and 30 dpi (n = 5 per group) in IKK2^fl/fl Sftpc-Cre mice and littermate controls. g Confocal microscopy of lung sections stained for E-cadherin (red) and CCL2 (green) in IKK2^fl/fl Sftpc-Cre mice and littermate controls. AMs alveolar macrophages, Neuts neutrophils, RMs recruited macrophages. a 2-Way ANOVA with Bonferroni post test, b 1-way ANOVA with Tukey’s post test. c–f Student’s t-test was used to compare between groups per time point