Fig. 3

Protective effects of ONO-4641 treatment in the mouse model of airway inflammation after short-term CS exposure. a–c Representative images of hematoxylin–eosin-stained lung sections 3 days after air or cigarette smoke (CS) exposure in vehicle- or ONO-4641-treated mice (a); numbers of total cells, neutrophils, and macrophages (b); and the levels of TNF-α IL-6, IL-1β, CXCL1, and CXCL2 (c) in BALF 3 days after CS exposure are shown (n = 2 in air exposure group, n = 8 in each CS exposure group). Data are presented as the mean ± SEM. Mice were exposed to CS for 60 min/day for the indicated periods. ONO-4641 or vehicle was intragastrically injected at 0.3 mg/kg once a day at 1 h before CS exposure for indicated days. Arrowheads denote infiltrated cells. Data are presented as the mean ± SEM. *P < 0.05. **P < 0.01. Scale bar, 50 μm. d, e Mice were exposed to room air or CS for 3 consecutive days with an additional 3 days of exposure to room air or CS. At days 4 to 6, vehicle or ONO-4641 was administered at 0.3 mg/kg once a day at 1 h before CS exposure for the indicated days in the Figure. The numbers of total cells, neutrophils, and macrophages (d) and the levels of TNF-α IL-6, IL-1β, CXCL1, and CXCL2 (e) in BALF at day 6 of the experiment are shown. (n = 4−6 in each group). Data are presented as the mean ± SEM. *P < 0.05