Fig. 3

GRP inhibitor, NSC77427, reduces lung pathology after influenza PR8 challenge. WT C57BL/6J mice (5 mice/treatment group) were infected i.n. with mouse-adapted influenza strain PR8 (LD₉₀; ~7500 TCID₅₀). Mice received vehicle (saline + 0.0096% DMSO) or GRP inhibitor (NSC77427; 20 μM/mouse; i.v.) daily from days 2 to 6 post-infection. On day 7 post-infection, mice were euthanized and lungs were extracted, fixed, and stained for histopathology. Photomicrographs of representative sections were taken at ×10 (a, b–d), and at ×40 (c). All scale bars are 100 μm. N = 4 mice/group. Asterisk indicates the pleural surface. e Quantitation of lung injury is based on the scoring system detailed in the Materials and methods section. Data shown are mean ± SD. *p = 0.002