Fig. 4 | Mucosal Immunology

Fig. 4

From: E. coli Nissle 1917 is a safe mucosal delivery vector for a birch-grass pollen chimera to prevent allergic poly-sensitization

Fig. 4

Intranasal pretreatment with EcN-Chim showed high levels of FOXp3, TGFβ, and IL-10 mRNA in bronchial lymph nodes. Total RNA was extracted from bronchial lymph nodes of mice treated with probiotic bacteria and sensitized/allergic controls at the end of the experiment and reverse-transcribed into cDNA. Expression of FOXp3, TGFβ, and IL-10 mRNA was measured by RT-PCR as described previously. Figure represents the ratio of the target genes to GAPDH, a FOXp3, b TGFβ and c IL-10. a−c represents mean ± SEM from pooled data of two independent experiments (total n = 10 mice per group). Error bars show mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Tukey’s Multiple Comparison Test

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