Table 2 Antibody response in serum and cytokine response in lung and spleen cells after pretreatment of EcN-Chim or EcN-Ctrl prior to poly-sensitization and challenge

From: E. coli Nissle 1917 is a safe mucosal delivery vector for a birch-grass pollen chimera to prevent allergic poly-sensitization

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  1. Female BALB/c mice were pretreated either orally or intranasally with EcN-Chim and EcN-Ctrl on every second day followed poly-sensitization and challenge with recombinant allergens. Mice were sacrificed 72 h after the last airway challenge. Serum samples were collected for antibody analysis. Spleens and lungs were collected for cytokine analysis from spleen cell culture and lung cell cultures stimulated with birch and grass pollen allergen. (a) IgG2a/IgE ratio represents in OD. (b) Lung (IL-5, IL-13) and (c) spleen (IL-5, IL-10, IFNγ cytokine levels are represented as pg/ml
  2. Data are representative of two independent experiments (n = 5 mice per group). Significant difference between allergic control group and treated group is indicated by alphabet (ä) and values are reported as mean ± SEM. äP < 0.05; ääP < 0.01; äääP < 0.001. The significant difference between the treated group is indicated by star/asterisk (*) and values are reported as mean ± SEM. *P < 0.05; **P < 0.01, ***P < 0.001 by Mann−Whitney U test in comparison to poly-sensitized control
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