Fig. 2

PSA-competent and PSA-deficient 9343 similarly reduce adenoma development. a–d SPF Min mice were inoculated sequentially (9343→86) or simultaneously (9343+86). Mice were killed at 12 weeks (macroadenomas) or 2 to 4 weeks (microadenomas). Bars indicate median and interquartile range. Macroadenomas: data shown are from 2 replicate experiments with 1 (Sham control only) to 11 mice in total per group. Microadenomas: data shown are from 1 experiment with 4 to 11 mice in total per group. a Images of representative methylene blue-stained distal colons. The 9343→86 and ΔPSA 9343→86 mice display decreased macroadenoma development compared with 9343+86 and 86 control mice. b Entire colon macroadenoma quantification from individual mice. Both 9343→86 and ΔPSA 9343→86 mice exhibit a similar reduction in macroadenomas in comparison with 9343+86 and 86 mice. Macroadenoma burden was similar between 9343 and ΔPSA 9343 mice. Image shows stained colons of 9343→86 high tumor producers: mouse #312 and mouse #238. c H&E colon sections displaying variable microadenoma histopathology of 9343→86 and 86 control mice. d Quantification of microadenomas from individual mice. The 9343→86 mice do not display a significantly lower microadenoma burden compared with 86 control mice. Multiple group comparisons were conducted using Kruskal–Wallis testing and pairwise comparisons using Mann–Whitney U testing