Fig. 5 | Mucosal Immunology

Fig. 5

From: Non-toxigenic Bacteroides fragilis (NTBF) administration reduces bacteria-driven chronic colitis and tumor development independent of polysaccharide A

Fig. 5

The 86 strain secretes an intraspecies inhibitory molecule. a Individual 9343, ΔPSA 9343, and 86 growth curves. Curves represent the average of triplicate values. b Co-inoculation assay of 9343 with 86 and ΔPSA 9343 with 86. Isolated B. fragilis colonies (10) plated from co-cultures were randomly selected and screened by strain-specific PCR. After equal part inoculation, 9343 and ΔPSA 9343 are no longer detected at 24 and 48 h. c Co-inoculation assay of 9343 with 86. After differential inoculation, 9343 is only detected at an input ratio of 90:10 (9343:86) at 24 and 48 h. d Agar diffusion assay of 9343 indicator strain (grown on the agar) with 86 supernatant (in wells). A zone of clearing (span of arrow) is induced by the 86 supernatant. Protease treatment with trypsin and proteinase-K and heat treatment at 100 °C impairs 86 supernatant activity. Top row: 86 wild-type (wt) supernatant; bottom row: 86 BFT mutant (mut) supernatant

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