Fig. 6

Therapeutic treatment with 9343 does not improve the health of 86-colonized mice. a–d SPF WT mice colonized with 86 or 9343 for 2 weeks were treated with 9343 or 86, respectively (86→9343^R and 9343→86^R). Superscript ^R denotes repeated treatments for 9 days. Mice were rested for 2 days before killing. Data shown include a single experiment with 4 to 5 mice per group. Bars indicate median and interquartile range. a Strain quantification measured by stool qPCR at the time of killing. Symbols represent individual mice. Diamonds: 3 of 5 86→9343^R mice establish detectable levels (10²–10⁶ copies) of treatment strain 9343 (mouse ID: 62, 61, 59). Triangles: 0 of 5 9343→86^R mice exhibit detectable levels of challenge strain 86. b Representative images of cecum and spleen: 86→PBS^R control and 86→9343^R ceca are small, pale, and contracted; spleens are enlarged. The 9343→PBS^R control and 9343→86^R ceca are comparable to Sham→PBS^R mice (not shown) in size, coloring, and content; spleens are not enlarged. c Mouse cecum weights. The 86→9343^R cecum weights are comparable to 86→PBS^R controls, indicative of disease. The 9343→86^R cecum weights are higher compared to 86→PBS^R control and 86→9343^R mice, indicative of protection. d Mouse spleen weights. The 86→9343^R spleen weights are higher compared to 9343→86^R mice but comparable to 86→PBS^R controls, indicative of disease. The 9343→86^R spleen weights are comparable to 9343→PBS^R controls, indicative of protection