Fig. 1

cDC2 play a central role in mediating the adjuvant activity of CDG. a Flow cytometry analysis of MPYS expression in lung DC subsets. n > 3. b Absolute number of lung DC subsets in C57BL/6 mice administered (i.n.) with saline or 5 µg FITC-CDG for 5 h. n = 3. c Absolute number of CD86⁺ lung DC in mice administered (i.n.) with saline or 5 µg CDG for 16 h. n > 3. d Absolute number of CCR7⁺ lung DC in mice administered (i.n.) with saline or 5 µg CDG for 16 h. n > 3. e–g Flow cytometry analysis of pRelA and pRelB in cDC1 (e), cDC2 (f), and moDCs (g) from mice treated with saline or CDG for 16 h. n > 3. h C57BL/6, Batf3^−/−, and IRF4^fl/flCD11c^cre mice were immunized (i.n.) with two doses (14 days apart) of PspA or PspA plus CDG (5 μg). Anti-PspA IgG in serum and IgA in BALF were determined by ELISA. n > 3. i IRF4^fl/flCD11c^cre mice were adoptively transferred (i.n.) with lung cDC2 sorted from WT mice lung and immunized (i.n.) with PspA or CDG/PspA. Serum anti-PspA IgG and BALF IgA were determined by ELISA. n = 3. Graphs represent means ± standard error from three independent experiments. The significance is represented by an asterisk (*) where p < 0.05 (unpaired Student’s t-test)