Fig. 6
CDG activates TNFR2−/− cDC2 in vivo to produce TNF. a, b Indicated mice were administered (i.n.) with saline or CDG for 5 h. TNF production was measured in lung homogenates by ELISA. n > 3. c WT and TNFR2−/− mice were treated with saline or CDG for 16 h. TNF in lung cDC2 was determined by an intracellular cytokine stain. n = 3. d TBK1fl/fl and TBK1fl/flVavcre mice were administered (i.n.) with saline or CDG for 5 h. TNF production was measured in lung homogenates by ELISA. n = 3. e Flow cytometry analysis of p-TBK1 expression in lung cDC2 from WT and TNFR2−/− mice treated with saline or CDG for 16 h. n = 3. Graphs represent means ± standard error from three independent experiments. The significance is represented by an asterisk (*) where p < 0.05 (unpaired Student’s t-test)
