Fig. 6

GITR is upregulated on activated CD4 and CD8 T cells in the mLN and the lung following influenza infection. Wild-type C57BL/6 mice were infected i.n. with influenza A/HK-X31 and expression of GITR was determined in the mLN a, b and the lung c, d. a GITR expression on total CD4 T cell (green), CD44^hi CD69⁺ FoxP3⁻ CD4 T cell (blue), and CD25^hi FoxP3⁺ Treg (red) in the mLN at day 3 p.i. b GITR expression on total CD8 T cell (green), CD44^hi CD69⁺ CD8 T cell (blue) in the mLN at day 3 p.i. c GITR expression kinetics on total CD4 T cell (green), CD44^hi T-bet⁺ Th1 (blue), and CD25^hi FoxP3⁺ Treg (red) in the lung with representative staining at day 7 p.i. shown on the right. d GITR expression kinetics on total CD8 T cell (green), D^b/NP_366–374-specific (blue), and D^b/PA_224–233-specific CD8 T cell (red) with representative staining at day 5 p.i. shown on the right. Dashed lines represent baseline/naive expression level of GITR. dMFI refers to the MFI for the specific antibody stain minus the fluorescence minus one (FMO) control. Data points indicate mean ± SEM. Data from a, b are representative of two independent experiments, each with three mice. Data from c, d were pooled from two independent experiments with a total of six mice per time point