Fig. 7

GITRL expression is highest on monocyte-derived inflammatory APC subsets after influenza X31 infection. Wild-type C57BL/6 mice were infected i.n. with influenza A/HK-X31 and expression kinetics of GITRL determined in the lung and mLN. a Expression kinetics of GITRL on different cell subsets from the lung defined in Supplementary Fig. S2a between day −1 and 8 p.i. with representative staining from day 5 p.i. shown below the kinetics plot. Gray filled histograms represent FMO and open histograms the GITRL staining. b Expression kinetics of GITRL on different cell subsets from the mLN defined in Supplementary Fig. S2b between day −1 and 8 p.i. with representative staining shown at day 2 p.i., with GITRL staining shown as open histograms and FMO as gray filled histograms. Horizontal dashed lines in the kinetics plots in a and b indicate the baseline/naive expression level of GITRL. c Total number of InfMF and InfDC in the lung between day 2 and 8 p.i. dMFI refers to the MFI for the specific antibody stain minus the FMO control. Data points indicate mean ± SEM. Data in a–c were pooled from two independent experiments with a total of 6–12 mice per time point. IntMF interstitial macrophages; AlvMF alveolar macrophages; InfMF inflammatory macrophages; InfDC inflammatory DCs; Subcap MF subcapsular sinus macrophages; Medul MF medullary macrophages