Fig. 7

Mice treated with a CCR2 antagonist have altered cytokine profiles and increased IL-17 expression following H1N1/MRSA dual infection. WT mice were treated with CCR2 antagonist (50 μg, subcutaneously) twice daily for 5 days during infection with H1N1. After 5 days of H1N1 infection mice were subsequently infected with MRSA for 24 h. a Lung bacterial burden was calculated by serial dilution of whole lung homogenates 24 h after MRSA infection. b Following MRSA infection, BAL was performed and numbers of leukocytes were quantified by cytospin of BALF and differential stain. c Lungs were collected and the expression of the indicated transcript was detected by qRT-PCR. Statistical significance was calculated with ANOVA b or Student’s t-test a, c, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 n = 4 mice per group