Fig. 1 | Mucosal Immunology

Fig. 1

From: Selective expression of constitutively activated STAT6 in intestinal epithelial cells promotes differentiation of secretory cells and protection against helminths

Fig. 1

Expression of STAT6vt protein leads to constitutive phosphorylation of STAT6 in intestinal epithelial cells of VillinCre_STAT6vt mice a, b Immunoblot of the intestine and lungs from naive VillinCre_STAT6vt mice and their control littermates (STAT6vt). Samples were analyzed for C-terminal FLAG tag a, total STAT6 (STAT6-M20, b, upper row), and phosphorylated STAT6 (pSTAT6, b, middle row). β-Actin and and α-Tubulin served as reference proteins. c Isolated intestinal epithelial cells from naive VillinCre_STAT6vt mice (left) and their control littermates (right) were stained for phosphorylated STAT6 (pSTAT6, orange) and the epithelial cell marker EpCAM (green). Blots are representative of one out of two independent experiments with two mice per group per experiment. Numbers indicate the molecular weight in kD. The picture shows one experiment with two mice per group. Scale bars represent 20 µm

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