Fig. 3

SCF expression during allergy and differential expression of 248SCF isoform. a Level of soluble (ECD) SCF in serum samples of asthmatic patients (n = 9) and non-asthmatics (n = 8) was measured by ELISA. b Soluble SCF in the serum of chronic allergic mice or naïve mice was measured by ELISA. c qPCR expression of the two isoforms SCF220 and SCF 248 in the lungs of allergic and naïve mice. d A monoclonal antibody against 248SCF was made using a synthetic peptide from exon 6. specifically binds to the full-length recombinant SCF protein that contains exon 6 but not the cleaved extracellular domain of SCF as indicated by direct ELISA. Data represents mean ± SE from each experiment with 4–5 replicates per experiment in the mouse studies. All data are representative of two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001