Fig. 4
From: Two distinct colonic CD14+ subsets characterized by single-cell RNA profiling in Crohn’s disease
CD64hiCD163− (P3) cells shape autologous CD4+ T cells towards a Th17/Th1 profile. a Gating strategy for sorting CD4+ T cells (purple gate), P3 (red gate) and P4 (green gate) cells in inflamed CD colon. b P3 and P4 cells were co-cultured with autologous colonic CD4+ T cells for 6 days. IL-17/IFN-γ expression was measured using intracytoplasmic staining after gating on CD3+ T cells (P3, n = 16; P4, n = 3) (Wilcoxon signed rank test). c Distribution of CCR6+CXCR3− (Th17), CCR6+CXCR3+ (Th17/Th1), CCR6−CXCR3+ (Th1) cells in MLN (n = 8) and colon (n = 12) of CD patients (Mann–Whitney test). d CCR6+CXCR3−CD62lowCD4+ cells isolated from MLN (diamond, n = 4) or inflamed CD colon (square, n = 3) were co-cultured with autologous P3 cells for 6 days. IL-17 and IFN-γ expression assessed using intracytoplasmic staining after gating on CD3+ T cells (Wilcoxon signed rank test)
