Fig. 7
T cell-mediated colitis development identified Ifi44 as a candidate gene. a Histological scoring of animals that received cells isolated from B6-Il10−/−, BC-R3-Il10−/− and BC-R2-Il10−/− mice (n = 6–12, **P ≤ 0.01, samples from two independent experiments). Representative H&E stained histological slides of the distal colon that received naive T cells isolated from B6-Il10−/−, BC-R3-Il10−/− and BC-R2-Il10−/− animals. b The gene expression levels of Tbx21, Rort, Ifn and Tnf measured in the proximal colon of B6-Rag1−/− animals compared with those in B6-Rag1−/− animals, which received cells from B6-Il10−/−, BC-R3-Il10−/− and BC-R2-Il10−/− mice. The relative quantification (RQ) of gene expression was measured by qPCR and referred to a reference sample set to 1 (n = 4-12; mean ± SEM; samples from two independent experiments). c The percentage of IL17A+, RORYt+, IFNγ+ and TNFα+ CD3+CD4+ T cells of animals, which received T cells isolated from B6-Il10−/−, BC-R3-Il10−/− or BC-R2-Il10−/− mice, was determined by flow cytometry (n = 3–9; mean ± SEM; * P ≤ 0.05, samples from two independent experiments). d Heat map of genes differently expressed due to the distal MMU3. Naive T cells from BC-R3-Il10−/− or BC-R2-Il10−/− mice were compared with B6-Il10−/− samples (n = 2, fold change > 2, intensity cutoff > 50). e Relative quantification (RQ) in gene expression of Ifi44 in colitogenic T cells is based on a reference sample set to 1 (n = 4–8, mean ± SEM; * = P < 0. 05, *** = < 0.001, samples from two independent experiments). f Densitometric quantification of IFI44 by western blot of lysates from naive T cells isolated from B6-Il10−/−, BC-R3-Il10−/− or BC-R2-Il10−/− normalized to the internal control GAPDH (n = 5). Representative western blot of IFI44 and GAPDH protein expression
