Fig. 1 | Mucosal Immunology

Fig. 1

From: Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism

Fig. 1

Induction of HMGB1 surface expression by platelets in response to exogenous and endogenous LTC4. Platelet-rich plasma (PRP) was obtained from the indicated mouse strains and stimulated with various agonists. The CD41+ platelet gate was analyzed by flow cytometry with Abs specific for HMGB1 (left panels) or CD62P (right panels) along with corresponding isotype controls. a Time-dependent inductions of surface HMGB1 and CD62P by LTC4, LTD4, and LTE4 at the indicated concentrations. b Comparison of LTC4 versus thrombin stimulation for surface expressions of HMGB1 and CD62P. c Effect of Cysltr2 deletion on LTC4-induced HMGB1 and CD62P expression by platelets. d Induction of HMGB1 and CD62P expression by platelet-derived LTC4 converted from exogenous LTA4. Results in a–d are mean ± SEM from three independent experiments that included a minimum of three mice per group

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