Fig. 2

Effects of RAGE or TLR4 blockade on LTC₄-induced platelet activation. Platelet- rich plasma (PRP) was obtained from the indicated mouse strains and stimulated with LTC₄ at the indicated concentrations or with thrombin (0.5 U/ml). Some samples were treated with the selective RAGE antagonist FPS-ZM1, and others with the TLR4 antagonist LPS-RS at the indicated doses prior to stimulation. a Effects of RAGE blockade on LTC₄-induced surface expression of HMGB1 (left) and CD62P (right) by CD41+ platelets stimulated for 30 min. b Effects of RAGE and TLR4 blockade on expression of HMGB1 (left) and CD62P (right) by platelets in PRP stimulated for 30 min by LTC₄ or thrombin. c Concentrations of CXCL7 (left) and TXB₂ (right) detected by ELISA in the supernatants from PRP stimulated as in b. d Effects of RAGE and TLR4 antagonists on surface expression of HMGB1 (left) and CD62P (right) by platelets in PRP stimulated by LTA₄ (to elicit conversion by platelet LTC₄S) or LTC₄ at the indicated doses. Results in a–d are mean ± SEM from three independent experiments that included a minimum of three mice per group