Fig. 3
Effects of RAGE blockade, HMGB1 neutralization, and CysLT2R antagonism on physiologic response of AERD-like Ptges−/− mice to Lys-ASA inhalation challenge. Df-primed Ptges−/− mice were treated with the indicated Abs, antagonists, or corresponding isotype and vehicle controls. Twenty-four hours later, mice were anaesthetized, sedated, mechanically ventilated, and challenged by aerosoled Lys-ASA or PBS control. a Maximum change in RL for the indicate groups of mice monitored for 45 min after Lys-ASA challenge. b Levels of HMGB1 collected in BAL fluids from the same mice as in a. c Levels of cysLTs in the BAL fluids. d Levels of CXCL7 in BAL fluids. e Levels of MMCP-1, f histamine and g PGD2 in the BAL fluids from the same mice as in a–d. Results are mean ± SEM from two independent experiments using a total of 10 mice in each group
