Fig. 4

Recruited platelets account for the Lys-ASA-induced increment in HMGB1. Lungs were collected from PBS- or Lys-ASA-challenged Ptges^−/− mice treated with a platelet-depleting anti-CD41 Ab or isotype control. a The lung sections were stained with anti-CD41 mAb or an isotype control. Infiltrating platelets are identified by the red staining. b Western blotting of lung lysates from Ptges^−/− mice of the indicated treatment groups for CD61 as a surrogate marker for recuited platelets. A blot from a representative individual experiment (top) and quantitative densitometry from two separate experiments (bottom) are shown. c BAL fluid levels of HMGB1 from mice challenged with Lys-ASA or PBS after treatment with anti-CD41 or isotype. Results in a are from single mice representative of at least five per group in three different experiments. Results in c are mean ± SEM from at least 10 mice per group from two separate experiments