Fig. 5

Rapid platelet-dependent increase in lung IL-33 induced by Lys-ASA challenge. Df-primed Ptges^−/− mice were treated with the indicated Abs, antagonists, or corresponding isotype and vehicle controls. a Levels of IL-33 (left), IL-5 (middle), and IL-13 (right) proteins detected in homogenates of lungs from the indicated groups of Ptges^−/− mice with and without platelet depletion collected 45 min after PBS- or Lys-ASA challenge. b Effects of treatment with the indicated Abs and antagonists on Lys-ASA-induced increases in IL-33, IL-5, and IL-13 detected in lysates of lungs from Ptges^−/− mice. c Western blotting for IL-33 protein in supernatants and pellets of washed platelets from naive WT mice subjected to the indicated stimuli for 1 h. Results in a and b are mean ± SEM from at least 10 mice per group from two separate experiments. Results in c are representative of three different experiments. Results from experiments using Ptges^−/− platelets yielded similar results