Fig. 1
From: The EBI2-oxysterol axis promotes the development of intestinal lymphoid structures and colitis
High EBI2 surface expression in human intestinal lymphocytes and upregulated gene expression of EBI2 and oxysterol-synthesizing enzymes in inflamed intestinal tissue. (a) EBI2 expression on human LPMCs of colon resections shown as mean fluorescence intensity ratio (MFIR) in naïve (CD19+CD27−) and memory (CD19+CD27+) subsets of B cells and in naïve (CD45RA+) and memory (CD45RA−) CD4+ and CD8+ T cells determined by FACS analysis. (b) Percentages of memory CD4+ T cells expressing EBI2 on human PBMCs from healthy volunteers (HV) and CD and UC patients and on human LPMCs of colon resections determined by FACS analysis. (c) Data from a human whole genome microarray (GEO data sets: GDS3268) was analyzed regarding mRNA expression levels of EBI2, CH25H and CYP7B1 in non-inflamed colon tissue of healthy volunteers (n = 63) and non-inflamed (n = 61) and inflamed (n = 62) colon tissue of UC patients. The dotted line represents the mean of healthy tissue (set to 1). (d) mRNA expression levels from rectal biopsies of UC patients, either non-inflamed from patients with quiescent disease activity (n = 24) or inflamed from patients with moderate to severe disease activity (n = 20) from the Swiss IBD cohort study were determined by RT-PCR and normalized to GAPDH using the ΔΔCt method. Data shown as mean ± SEM. Statistical analysis: (a): One-way ANOVA with Dunnetts test; (b-d): Mann–Whitney U test; *p < 0.05, ***p < 0.001, ****p < 0.0001
