Fig. 1

JAM-A expression is not necessary for PMN migration in response to various pro-inflammatory stimuli. a, b Number of PMN in the peritoneal lavage in mice untreated (control) or 2 h post-injection with zymosan by flow cytometry. a Jam-a^fl/fl versus LysM-Cre⁺;Jam-a^fl/fl mice. b Jam-a^WT versus Jam-a^KO mice. Symbols represent individual mice. Data are pooled from four independent experiments and represent means ± SEM. ***P < 0.001 by two-way analysis of variance (ANOVA) with a Bonferroni multiple comparison post hoc test. ns: not significant. c, d Number of PMN in the peritoneal lavage 2 h post-injection with LPS (c) or 4 h post-injection with TNFα (d) by flow cytometry. Each data point represents individual mice from two independent experiments. Data represent means ± SEM. **P < 0.01 by two-way ANOVA with a Bonferroni multiple comparison post hoc test. ns: not significant. e, f Chemotactic migration of bone marrow neutrophils across collagen type I coated polycarbonate filters (5-μm pore size) towards a gradient of CXCL1 or LTB₄. e Jam-a^fl/fl versus LysM-Cre⁺;Jam-a^fl/fl mice. f Jam-a^WT versus Jam-a^KO mice. Results are represented as the percentage of PMN that migrated into the bottom chambers that has been normalized to controls (Jam-a WT and Jam-a^fl/fl, respectively). Each dot represents the value for an individual experiment (N = 6 independent experiments ± SEM). Differences are not significant (ns) by two-way ANOVA with a Bonferroni multiple comparison post hoc test. PMN polymorphonuclear neutrophils, JAM-A Junctional adhesion molecule-A, LPS lipopolysaccharide