Fig. 2

CXCL1 production is reduced in Jam-a^KO mice while unaffected in LysM-Cre⁺;Jam-a^fl/fl mice. a, b Quantitative measurement of CXCL1 expression in vivo by ELISA in peritoneal exudates from untreated mice or after 2 h post-injection of zymosan. a Jam-a^WT versus Jam-a^KO mice. Symbols represent individual mice. Data are pooled from four independent experiments and represent means ± SEM. ***P < 0.001 by two-way analysis of variance (ANOVA) with a Bonferroni multiple comparison post hoc test. ns: not significant. b Jam-a^fl/fl versus LysM-Cre⁺;Jam-a^fl/fl mice. Symbols represent individual mice and data are pooled from four independent experiments. Data represent means ± SEM. Differences are not significant by two-way ANOVA with a Bonferroni multiple comparison post hoc test. c, d Quantitative measurement of CXCL1 expression by ELISA in cell media supernatants of isolated peritoneal macrophages from mice either untreated or treated ex vivo with zymosan for 1 h. c Jam-a^WT versus Jam-a^KO mice mice. Symbols represent individual mice from three independent experiments. Data represent means ± SEM. ***P < 0.001 by two-way ANOVA with a Bonferroni multiple comparison post hoc test. d Jam-a^fl/fl versus LysM-Cre⁺;Jam-a^fl/fl mice. Symbols represent individual mice from two independent experiments. Data represent means ± SEM. Differences are not significant (ns) by two-way ANOVA with a Bonferroni multiple comparison post hoc test