Fig. 4

Impaired NF-kB activation in peritoneal macrophages from Jam-a^KO and Villin-Cre⁺;Jam-a^fl/fl mice in response to zymosan and LPS. Peritoneal macrophages from Jam-a^KO, Villin-Cre⁺;Jam-a^fl/fl, LysM-Cre⁺;Jam-a^fl/fl and controls were either untreated or stimulated with zymosan for 5 and 10 min (a–c) or with LPS for 15 and 30 min (d–f). Protein cell lysates were collected, separated by SDS-PAGE and immunoblotted for NF-kB p65 pSer536, NF-kB p65 total, and GAPDH as a loading control. Data are a representative immunoblots from three independent experiments. Graphs represent densiometric analysis for values of NF-kB p65 pSer536 normalized to p65 total. Data are means ± SEM of three independent experiments (3–5 mice per group). *P < 0.05, **P < 0.01 by two-way ANOVA with a Bonferroni multiple comparison post hoc test. LPS lipopolysaccharide, NF-kB nuclear factor- kB, SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis