Fig. 3 | Mucosal Immunology

Fig. 3

From: Mucosal boosting of H56:CAF01 immunization promotes lung-localized T cells and an accelerated pulmonary response to Mycobacterium tuberculosis infection without enhancing vaccine protection

Fig. 3

Mucosal H56:CAF01 boosting enhances the magnitude, but not phenotype, of the early vaccine CD4 T cell response to pulmonary Mtb infection. CB6F1 mice were rested 6 weeks after vaccination and challenged with a low dose of aerosolized Mtb. a The number of H56-specific CD4 T cells in lung parenchyma (IV−) by ICS for expression of any of the cytokines IFNγ/TNF-α/IL-2/IL-17 over the course of infection. b The proportion of H56-specific cells from (a) expressing each individual cytokine is shown for 3 weeks (left) and 6 weeks (right) after Mtb challenge. c Combinatorial Boolean analysis of H56 lung CD4 T cells (from (b)) at 3 weeks post Mtb challenge are shown. Bar/symbol, mean ± SD. *p < 0.05, **p < 0.01 vs. Ctrl or as indicated via ANOVA with Tukey’s posttest of log-transformed data (a) or raw percentage (b)

Back to article page