Fig. 3
Induction of autophagy limits induction of IL-23 and Il-1β in macrophages. Bone marrow-derived macrophages (BMDMs) prepared from wild-type mice were pre-incubated with rapamycin at 100 nM for 2–12 h and then stimulated with LPS (10 ng/ml) for 3 h. qPCR analysis for the expression of IL-23 (a) and IL-1β (b); c Western blot analysis for the levels of LC3B-1/B-II, p62 and GAPDH in BMDMs treated with rapamycin at 100 nM for 2–18 h; d Quantification of LC3B-1/II and p62 in comparison to GAPDH control via densitometry; e, f BMDMs were prepared from control (Atg7f/f) and Cx3cr1-cre;Atg7f/f mice and stimulated with LPS (10 ng/ml) for 3 h. qPCR analysis for the expression of IL-23 and IL-1β; Data are presented as mean ± SEM (t-test) of at least n = 4 experiments, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001
