Fig. 4
Inflammatory and fibrotic responses in Cx3cr1-cremTORf/f and Cx3cr1-creAtg7f/f mice. Histological analysis of colon fibrosis in CX3cr1, CX3cr1mTORf/f, and CX3cr1Atg7f/f mice. Representative images of myofibroblast staining with anti-αSMA antibody (a) and collagen staining with Trichrome blue (c); Quantification of the thickness of the submucosal αSMA-positive layer (b) and average intensity of Trichrome blue staining (d); e, f FACS analysis and quantification of αSMA-positive cells in the colon; g qPCR analysis of fibrosis markers and cytokines; h Expression of IL-23, IL-1β, and TNFα in purified CX3Cr1+ mononuclear phagocytes in mouse colon samples measured by qPCR. Data are presented as mean ± SEM. n = 5, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001
