Fig. 5
IFN-γ promotes cell migration potential. a, b Representative images of hNECs migrating through Transwell inserts toward serum for 48 h. Cells were cultured with IFN-γ (100 ng/ml) alone or in combination with inhibitors. Cells that migrated to the lower surface of the filters were quantified. Photographs were taken at ×40 magnification and are presented in the bar graph. Statistical significance was determined by Kruskal–Wallis tests (P < 0.05), followed by Mann–Whitney U-test for pairwise comparisons. **P < 0.01. Data are shown as means ± s.e.m (n = 3). c Representative F-actin (red) images of hNECs and MTT assay results (d) under resting conditions or stimulated with IFN-γ alone or in combination with p38, ERK inhibitors for 48 h. Scale bars, 50 μm. Statistical significance was determined by Kruskal–Wallis tests (P > 0.05), followed by Mann–Whitney U-test for pairwise comparisons. n.s. not significant. Data are shown as means ± s.e.m (n = 5). e The transition of TEER was measured after treatment of IFN-γ (100 ng/ml) alone or in combination with inhibitor mixtures. Resistance was measured at 24 h intervals from 0 to 72 h. Statistical significance was determined by Kruskal–Wallis tests (P < 0.05), followed by Mann–Whitney U-test for pairwise comparisons in each time point. Significant differences are denoted relative to vehicle (*P < 0.05, **P < 0.01) or to the IFN-γ + inhibitor mixture treatment group (#P < 0.05, ##P < 0.01). Data are shown as means ± s.e.m (n = 3)
