Fig. 3 | Mucosal Immunology

Fig. 3

From: Alcohol-induced IL-17A production in Paneth cells amplifies endoplasmic reticulum stress, apoptosis, and inflammasome-IL-18 activation in the proximal small intestine in mice

Fig. 3

Alcohol induces IL17A expression in Paneth cells but not Th17 cells in the proximal small intestine. a Representative images and (b) quantification of IL-17A immunohistochemistry (IHC) in 10 day EtOH 9 h binge alcohol-fed and pair-fed (PF) mice. c IL-17A from whole PSI protein was also quantified (d) by Western blot. e Flow cytometry of small intestinal lamina propria (SI LP) detected Th17 cells, defined as CD45+, TCRb+, CD4+, IL-17+ live single cells. f Isolated small intestinal crypts were stimulated ex vivo with various concentrations of ethanol (0, 4, 25, 50, and 100 mM) for 2, 4, 6, or 8 h and IL-17A was measured from the supernatants by ELISA. n = 5–7 mice/group; *p < 0.05

Back to article page