Fig. 5

Alcohol administration induces pro-apoptotic signaling and ER stress in the proximal small intestine. a Pro-apoptotic proteins Bax, Bim, Bid, truncated Bid (tBid) and cleaved (clv) PARP, as well as ER stress related proteins CHOP, p-eIF2α, eIF2 α, GADD34 full length (fl) were measured by Western blot and quantified (b) from PSI tissue of pair-fed (PF) mice, as well as mice fed a 10-day chronic alcohol, a 10 day EtOH plus 4 h binge or a 10 day EtOH 9 h binge diet. c Caspase-3 (fl), cleaved (clv) caspase-3, caspase-2, and caspase-8 p43 were measured from the same PSI samples by Western blot and quantified (d). e Mice received either calorie matched diet (PF) or 10 day EtOH 9 h binge and some mice from each group also received in vivo administration of the ER stress inhibitor 4-phenylbutiric acid (4-PBA). Expression in the PSI of CHOP, pro-caspase-8 and clv caspase-8 p43 were measured by Western blot and quantified (f). For A-D, n = 5–7 mice/group; p < 0.05 * vs. pair-fed (PF), # vs. 10 day EtOH no binge, † vs. 10 day EtOH 4 h binge. For e–f, n = 5–7 mice/group; p < 0.05 * vs. pair-fed, # vs. 10 day EtOH no binge, † vs. 10 day EtOH 4 h binge, ° vs. 10 day EtOH 9 h binge