Fig. 6 | Mucosal Immunology

Fig. 6

From: Alcohol-induced IL-17A production in Paneth cells amplifies endoplasmic reticulum stress, apoptosis, and inflammasome-IL-18 activation in the proximal small intestine in mice

Fig. 6

Alcohol-induced CHOP expression localizes to Paneth cells. a PSI epithelial and immune cell fractions (intraepithelial [IEL] and lamina propria leukocytes [LPL]) were isolated from pair-fed (PF) and 10 day EtOH 9 h binge mice for Western blot analysis and quantified (b) CHOP and Bax proteins. c Representative CHOP immunofluorescent (IF) images of PSI samples of pair-fed (PF) and 10 day EtOH 9 h binge mice. d Insets show individual crypts and the more intense IF staining observed in the 10-day +9 h binge alcohol samples. e Crypts were isolated from PF and 10 day EtOH 9 h binge mice and Bax and Bim proteins were measured by Western blot and quantified (f). n = 3–7 mice/group; *p < 0.05 vs. pair-fed. Scale bars = 50 µm

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