Fig. 7 | Mucosal Immunology

Fig. 7

From: Alcohol-induced IL-17A production in Paneth cells amplifies endoplasmic reticulum stress, apoptosis, and inflammasome-IL-18 activation in the proximal small intestine in mice

Fig. 7

Tight-junction levels in the proximal small intestine are reduced by alcohol and restored by in vivo IL-17 blockade. a The bacterial cell wall component LPS was measured in the livers of pair-fed (PF) and 10 day EtOH 9 h binge mice, as well as EtOH mice treated with anti-IL-17 antibody or an isotype control. b ZO-1 immunofluorescent (IF) staining was completed on the PSI of these same mice and quantified. c Occludin was measured from the PSI by Western blot and quantified (d). mRNA expression of multiple tight junction proteins including Claudin-1, Claudin-5, and Claudin-15, Occludin and ZO-1 were measured by qPCR from PSI. For a, n = 5–8 mice/group. For B, n = 6 mice/group and 5 images/mouse were quantified. For c–d, n = 6 mice/group. For e, n = 7–10 mice/group. *p < 0.05 vs. pair-fed (PF)

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