Fig. 10
From: JunB plays a crucial role in development of regulatory T cells by promoting IL-2 signaling
IL-2-induced expansion of Treg cells prevents Junbfl/flCd4-Cre mice from exacerbation of DSS-induced colitis. a, b Control IgG or IL-2-anti-IL-2 complex was injected to Junbfl/flCd4-Cre mice on days 0, 1, and 2, and cells from the indicated tissues were analyzed by flow cytometry on day 5. Representative results of flow cytometry (a) and the average percentages of Foxp3+ cells among CD4+ T cells (b) are shown. Results are means ± SEMs (n = 4 mice per each group). c Junbfl/flCd4-Cre mice were administrated with 2.0% DSS in drinking water for 5 days, and then they were given by normal water in the following days. Control IgG or IL-2-anti-IL-2 complex was injected on days 0, 1, 2, 3, and 4. The average of body weight is shown as percentage relative to the initial value. Results are means ± SEMs (n = 7 or 8 mice per each group). d, e Junbfl/flCd4-Cre mice were treated as in (a), and colonic lamina propria cells were analyzed by flow cytometry on day 5. Representative results of flow cytometry (d) and the average percentages of IL-17A+ cells among CD4+ T cells (e) are shown. Results are means ± SEMs (n = 4 mice per each group). *p < 0.05, **p < 0.01, ****p < 0.0001, n.s. not significant (two-tailed Student’s unpaired t test)
