Fig. 9
From: JunB plays a crucial role in development of regulatory T cells by promoting IL-2 signaling
Production of IL-2 in T cells is reduced in Junbfl/flCd4-Cre mice. a, b Cells from the indicated tissues of control or Junbfl/flCd4-Cre mice were analyzed by flow cytometry. Representative results of flow cytometry are shown in Supplementary Figure 5. The average percentages of IL-2+ cells among CD4+ T cells (a) and CD4−T cells (CD8+ T cells) (b) are shown. Results are means ± SEMs (n = 6–8 mice per each genotype). c Schematic diagram for generation of bone marrow (BM) chimera mice. BM cells from wild type (CD45.1) and Junbfl/flCd4-Cre (CD45.2) mice were mixed at a ratio of 1:1, and transferred to a recipient wild-type mouse (CD45.1) that had been irradiated at 8.5 Gy. d, e Eight weeks after the BM transfer, cells from the indicated tissues were analyzed by flow cytometry. The average percentages of Foxp3+ cells among CD4+ T cells (d) and CD4+TCRβ+ cells among total CD45+ cells (e) are shown. Results are means ± SEMs (n = 7 mice). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. not significant (two-tailed Student’s unpaired t test)
