Fig. 1
Increased IL-17 and diminished IFNγ production from early Tbx21−/− effectors primed by IAV. 1 × 106 naïve WT or Tbx21−/− OT-II cells were adoptively transferred to congenic (CD45.1+) WT hosts and challenged with a sublethal dose of PR8-OVAII. At 5 dpi, dLNs were harvested and cytokine production assessed from donor cells by intracellular staining. a Representative staining for IL-2 from WT (black) and Tbx21−/− (grey) cells and control staining of WT cells without re-stimulation (solid) along with a summary of the percent of IL-2 positive cells (left) and MFI (right) for each population (n = 4 mice/group). b IFNγ production with representative staining (left) and the percent of positive cells (right). c Representative staining for IL-17 from WT (left) and Tbx21−/− (right) cells with summary from 4 mice/group, and (d) representative staining of IL-17 vs. IFNγ from Tbx21−/− cells. Results presented from 1 of 2 similar experiments
