Fig. 3
T-bet controls diverse aspects of cytokine production by effector cells primed by IAV. a Representative staining for stated combinations of cytokines are shown for donor cells in the lung at 7 dpi with (b) summary analysis for individual cytokine production from 4 mice/group. c Representative staining for granzyme B is shown for WT (black) and Tbx21−/− cells (grey) in the lungs vs. WT cells in the spleen (solid), with MFI analysis from 3 mice per group (dotted line represents average spleen MFI for granzyme B). Results representative of 3 similar experiments. d Donor effectors in the lung were also analyzed for expression of transcription factors T-bet, Rorγt, GATA3, and FoxP3 at 7 dpi, with the frequency of positive donors cells shown from 4 mice/group. Representative staining of host CD4 T cells for FoxP3 and CD25 is also shown as a staining control. One of 3 separate experiments. e Follicular helper cells (TFH) within WT and Tbx21−/− donor cells at 7 dpi was determined as depicted using CXCR5, PD1, and Bcl-6, with TFH frequency shown for the spleen (left panel) and dLN (right panel) from 4 mice per group. 1 of 2 separate experiments
