Fig. 7

Tbx21^−/− CD4 cells outcompete WT cells for memory niches following IAV priming. Representative staining (left, histogram) and summary analysis of lung effectors from groups of 4 mice receiving OT-II donor cells at 7 dpi (left bar graph) or from analysis of NP₃₁₁⁺ polyclonal cells responding in bone marrow chimeras at 8 dpi from 3 mice per group (right bar graph) for (a) Ly6C, (b) CD127, and (c) CD25. 1 × 10⁶ naïve WT and Tbx21^−/− OT-II cells were co-transferred to CD45.1⁺ hosts then challenged with sublethal PR8-OVA_II. d The frequency of WT and Tbx21^−/− cells within the donor gate at 28 dpi (4 mice/group). e The MFI of CD127 expression by lung donor cells is shown at 28 dpi from 4 mice/group. f Summary analysis of IFNγ, IL-17, and IL-2 production from co-transferred WT and Tbx21^−/− OT-II cells in the lungs at 28 dpi from 4 mice. At 28 dpi, adoptive hosts were given fluorescent anti-CD4 antibody i.v. and organs harvested 5 min later. g Shown is representative staining of Tbx21^−/− donor cells in the spleen (open) and lung (shaded) for CD69 (left histogram) and CD127 (right histogram), as well as lung for donor cell protection from the i.v. administered anti-CD4 antibody. h The frequency of CD69^high WT (black) and Tbx21^−/− (open) OT-II cells in the lung and spleen is shown at 28 dpi from 3 mice/group. Results representative of two independent experiments