Fig. 1

Up-regulation of Eftud2 expression in CAC lesions as well as macrophage infiltrates. a Schematic overview of CAC protocols. In brief, mice were injected intraperitoneally with AOM (10 mg/kg). After 5 days, these rodents received drinking water containing 1.5% DSS for 1 week. Thereafter, mice were maintained on regular water for 2 weeks and received three more DSS treatment cycles. b On day 100 after CAC induction, Eftud2 expression in the colons was examined by Western blotting. Each lane represents one mouse. c Eftud2 expression in the tumor (T) and non-tumor (N) colon tissues of CAC-bearing mice was examined by Western blotting. The number indicates one mouse. d On day 15 after colitis induction, Eftud2 expression in the colons was examined by Western blotting. Each lane represents one mouse. e On day 100 following CAC induction, immune cell subsets in the LP of colons of MKO and WT mice were fractionated by FACS sorting and Eftud2 expression was examined by quantitative RT-PCR. Five pairs of mice were analyzed. f On day 0, 35, and 100 after CAC induction, the colon tissues of MKO and WT mice were dissected, respectively, and CD68 as well as Eftud2 staining was examined by immunofluorescence. Five pairs of mice were examined. An example is shown. Magnification: ×200. ***P < 0.001