Fig. 3

Loss of Eftud2 in myeloid cells attenuates chronic intestinal inflammation. a CAC models were established in MKO and WT littermates (the number is 20, respectively). Survival rate was addressed by Kaplan–Meier survival analysis. b, c On day 35 after CAC induction, the colons of MKO and WT mice were dissected. H&E staining was performed. Magnification: ×200 (b). The extent of colitis was scored (c). Five pairs of mice were examined. An example is shown. d On day 35 and 100 following CAC induction, the colons of MKO and WT mice were dissected and cultured for 24 h. The supernatants were collected and cytokine levels indicated were determined by ELISA. Each group consists of 6–8 mice. e RNA from the colons of MKO and WT mice was extracted and cytokine as well as mediator levels indicated were examined by quantitative RT-PCR. Each group consists of 6-8 mice. f On day 35 and 100 after CAC induction, the colonic tissues of MKO and WT mice were dissected and CEC were isolated as described in Methods. Phosphorylated STAT3 expression in CEC was detected by Western blotting. Each lane represents one mouse. g BMDM were isolated from MKO and WT mice and cultured as described in Methods. Cells were then stimulated with LPS (100 ng/ml) for 12 and 24 h, respectively. IL-6 and TNF-α production were determined by ELISA. The data are pooled from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001