Fig. 6

Decreased LPS/TLR4 signaling pathway in MKO mice subjected to AOM/DSS. a On day 35 and 100 after CAC induction, the colons of MKO and WT mice were dissected and the expression of the kinases and proteins indicated involving TLR4 signaling in the colons was detected by Western blotting. Each lane represents one mouse. b BMDM were isolated from MKO and WT mice and treated with LPS (100 ng/ml) for 0–2 h and the activation of TLR4-NF-κB signaling was detected by Western blotting. An example of four independent experiments is shown