Fig. 1

Neutrophils interact with other cells as they migrate through granulomas. a Densely packed neutrophils are present in the space surrounding acellular caseum (indicated in DAPI) in a cynomolgus macaque granuloma. Scale bar = 200 μm. b Plotting the location of granulomas demonstrates neutrophils (blue) is present in multiple granuloma regions including the lung adjacent to granulomas, the lymphocyte cuff (CD3+ T cells; green) and are especially abundant between caseum and CD68+ epithelioid macrophages (red). c Neutrophils (pseudocolored magenta) and T cells (green) interact along immunologic synapse-like structures (white). Scale bar = 5 μm. d CD163+ macrophages (green) in the lymphocyte cuff phagocytose apoptotic-appearing calprotectin + neutrophils (blue, arrow). HAM56 staining is indicated in red. Scale bar = 10 μm. e Neutrophils accumulate at the caseum-epithelioid macrophage interface (stained with HAM56, red) where epithelioid macrophages can be seen phagocytosing neutrophils (blue, arrows). CD163+ lymphocyte cuff macrophages are indicated in green. Scale bar = 20 μm